However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing. for flowthrough chromatography because the relatively low binding capacities are. We previously showed that Capto MMC ImpRes mixed-mode chromatography under bind-elute mode can effectively remove this LC-missing species. Such secondgeneration highflowrate resins (e.g., Capto Q from GE. In addition, Capto MMC ImpRes and Capto adhere ImpRes have also both been. Capto MMC ImpRes is a mixed-mode resin based on the same ligand as that of Capto MMC, which possesses cation exchange and hydrophobic moieties, but has reduced bead size and ligand density, and hence offers improved resolution 4,5. While for bsAbs based on other platforms removal of such byproduct can pose considerable challenge to the downstream team, in this case WuXiBody's unique design makes separation relatively straightforward. Another example of an asymmetric bsAb is the antigen-binding fragment (Fab). English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian esk. For purifying a WuXiBody-based symmetric bsAb, we identified a LC-missing species as a major byproduct. Polishing of a Domain Antibody using Captoâ ¢ MMC ImpRes. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. (A) Dynamic binding capacity of Capto MMC at 1 min residence time for three different proteins at different conductivities. 8 1.0 M NaOH should only be used for cleaning purposes. Its key feature is the replacement of one parental antibody's CH1/CL region with the T cell receptor (TCR) constant domain, a design that promotes cognate heavy chain (HC)-light chain (LC) pairing. 7 Capto MMC can be used under cold-room conditions, but for some proteins the capacity may decrease. WuXiBody is a bispecific antibody (bsAb) platform developed by WuXi Biologics. In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove.
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